Effects of Transplantation and Microhabitat on Rhizosphere Microbial Communities during the Growth of American Ginseng

Transplanting has been widely used in American ginseng (Panax quinquefolium L.) cultivation Northwest China to mitigate the negative effects of continuous cropping obstacles. Because accumulation pathogenic microorganisms and change soil properties, transplanting newly cultivated fields after two years growth become a major planting pattern. Despite improving quality ginseng, properties microbiota on during process are poorly understood. In present study, microbial communities, physico-chemical morpho-physiological parameters were analyzed investigate characteristics both root microhabitats. Results indicated that structure species bacterial fungal communities changed significantly different microhabitats before transplantation. Moreover, assemblage community was dominated by deterministic processes. The stochastic ratio increased niche breadth decreased transplanting. While assembly process, there no significant difference NST, βNTI or Bacterial co-occurrence networks demonstrated higher connectivity but lower aggregation microhabitat, while remained stable Gammaproteobacteria biomarker Alphaproteobacteria, Betaproteobacteria Gemmatimonadetes biomarkers microhabitat. Sordariomycetes with high relative abundance functional important ASVs correlated pH, organic matter, total nitrogen, available phosphorus, potassium fresh weight, taproot diameter stem height ginseng. Partial least squares path modeling showed affected formation specific ASVs. microhabitat had positive effect growth, rare taxa effect. Our results provide good starting point for future studies succession influenced transplantation pattern